ELK8103-48T, Bb(Bilirubin) ELISA Kit, 48T

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ELK8103-96T, Bb(Bilirubin) ELISA Kit, 96T

2.475,20 RON

Bb(Bilirubin) ELISA Kit

SKU
ELK8103-96T

Alternative Names: Hematoidin

Species: General

Assay Type: Competitive Inhibition

Sensitivity: 0.45 µg/mL

Standard: 100 µg/mL

Detection range: 1.57-100 µg/mL

Sample type: serum, plasma and other biological fluids

Assay length: 2h

Research Area: Liver function

Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Bb protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Bb. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Bb in the samples is then determined by comparing the OD of the samples to the standard curve.

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Description

Alternative Names: Hematoidin

Species: General

Assay Type: Competitive Inhibition

Sensitivity: 0.45 µg/mL

Standard: 100 µg/mL

Detection range: 1.57-100 µg/mL

Sample type: serum, plasma and other biological fluids

Assay length: 2h

Research Area: Liver function

Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Bb protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Bb. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Bb in the samples is then determined by comparing the OD of the samples to the standard curve.