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ELK8080-96T, AED(Androstenediol) ELISA Kit, 96T
2.475,20 RON
AED(Androstenediol) ELISA Kit
SKU
ELK8080-96T
Alternative Names: 5-AED; 5AED; 5-Androstenediol; Androst-5-Ene-3Beta,17Beta-Diol
Species: General
Assay Type: Competitive Inhibition
Sensitivity: 53.4 pg/mL
Standard: 10000 pg/mL
Detection range: 156.25-10000 pg/mL
Sample type: serum, plasma and other biological fluids
Assay length: 2h
Research Area: Endocrinology;Reproductive science;Hormone metabolism;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with AED protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to AED. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of AED in the samples is then determined by comparing the OD of the samples to the standard curve.
| Price | 2.080,00 RON (preturile sunt fara TVA) |
|---|---|
| Description |
Alternative Names: 5-AED; 5AED; 5-Androstenediol; Androst-5-Ene-3Beta,17Beta-Diol Species: General Assay Type: Competitive Inhibition Sensitivity: 53.4 pg/mL Standard: 10000 pg/mL Detection range: 156.25-10000 pg/mL Sample type: serum, plasma and other biological fluids Assay length: 2h Research Area: Endocrinology;Reproductive science;Hormone metabolism; Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with AED protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to AED. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of AED in the samples is then determined by comparing the OD of the samples to the standard curve. |
