ELK8073-96T, Dog TMEM27(Transmembrane Protein 27) ELISA Kit, 96T

ELK8073-96T, Dog TMEM27(Transmembrane Protein 27) ELISA Kit, 96T

ELK8074-96T, LTA4(Leukotriene A4) ELISA Kit, 96T

ELK8074-96T, LTA4(Leukotriene A4) ELISA Kit, 96T

ELK8074-48T, LTA4(Leukotriene A4) ELISA Kit, 48T

1.814,75 RON

LTA4(Leukotriene A4) ELISA Kit

SKU
ELK8074-48T

Alternative Names: LT-A4

Species: General

Assay Type: Competitive Inhibition

Sensitivity: 46.3 pg/mL

Standard: 10000 pg/mL

Detection range: 156.25-10000 pg/mL

Sample type: serum, plasma, tissue homogenates, cell culture supernates and other biological fluids

Assay length: 2h

Research Area: Metabolic pathway;Infection immunity;Autoimmunity;

Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with LTA4 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to LTA4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of LTA4 in the samples is then determined by comparing the OD of the samples to the standard curve.

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Description

Alternative Names: LT-A4

Species: General

Assay Type: Competitive Inhibition

Sensitivity: 46.3 pg/mL

Standard: 10000 pg/mL

Detection range: 156.25-10000 pg/mL

Sample type: serum, plasma, tissue homogenates, cell culture supernates and other biological fluids

Assay length: 2h

Research Area: Metabolic pathway;Infection immunity;Autoimmunity;

Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with LTA4 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to LTA4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of LTA4 in the samples is then determined by comparing the OD of the samples to the standard curve.