ELK8071-48T, Human Anti-AsAb(Anti-Sperm Antibody) ELISA Kit, 48T

Alternative Names: Anti-Spermatozoa Antibodies; Sperm Antibodies; Antisperm Antibodies

Species: Human

Assay Type: Sandwich

Sensitivity: 1.17 ng/mL

Standard: 200 ng/mL

Detection range: 3.13-200 ng/mL

Sample type: serum, plasma and other biological fluids

Assay length: 3.5h

Research Area: Reproductive science;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Anti-AsAb. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Anti-AsAb. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human Anti-AsAb, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Anti-AsAb in the samples is then determined by comparing the OD of the samples to the standard curve.