ELK8068-48T, Mouse RETNLb(Resistin Like Beta) ELISA Kit, 48T

Alternative Names: XCP2; FIZZ2; HXCP2; CCRG; RETNL2; RELMb; Foundin In Flammatory Zone 2; Colon and small intestine-specific cysteine-rich protein; Colon carcinoma-related gene protein

Species: Mouse

Assay Type: Sandwich

Sensitivity: 2.54 pg/mL

Standard: 400 pg/mL

Detection range: 6.25-400 pg/mL

Sample type: serum, plasma and other biological fluids

Assay length: 3.5h

Research Area: Endocrinology;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse RETNLb. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse RETNLb. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse RETNLb, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse RETNLb in the samples is then determined by comparing the OD of the samples to the standard curve.