ELK8049-48T, Mouse CHEK1(Checkpoint Kinase 1) ELISA Kit, 48T

ELK8049-48T, Mouse CHEK1(Checkpoint Kinase 1) ELISA Kit, 48T

ELK8050-48T, Mouse GADD45g(Growth Arrest And DNA Damage Inducible Protein Gamma) ELISA Kit, 48T

ELK8050-48T, Mouse GADD45g(Growth Arrest And DNA Damage Inducible Protein Gamma) ELISA Kit, 48T

ELK8049-96T, Mouse CHEK1(Checkpoint Kinase 1) ELISA Kit, 96T

2.963,10 RON

Mouse CHEK1(Checkpoint Kinase 1) ELISA Kit

SKU
ELK8049-96T

Alternative Names: CHK1; Cell cycle checkpoint kinase; Serine/threonine-protein kinase Chk1

Species: Mouse

Assay Type: Sandwich

Sensitivity: 0.058 ng/mL

Standard: 10 ng/mL

Detection range: 0.16-10 ng/mL

Sample type: Tissue homogenates, cell lysates and other biological fluids.

Assay length: 3.5h

Research Area: Signal transduction;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse CHEK1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse CHEK1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse CHEK1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse CHEK1 in the samples is then determined by comparing the OD of the samples to the standard curve.

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Description

Alternative Names: CHK1; Cell cycle checkpoint kinase; Serine/threonine-protein kinase Chk1

Species: Mouse

Assay Type: Sandwich

Sensitivity: 0.058 ng/mL

Standard: 10 ng/mL

Detection range: 0.16-10 ng/mL

Sample type: Tissue homogenates, cell lysates and other biological fluids.

Assay length: 3.5h

Research Area: Signal transduction;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse CHEK1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse CHEK1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse CHEK1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse CHEK1 in the samples is then determined by comparing the OD of the samples to the standard curve.