ELK8020-48T, NAADP(Nicotinic Acid Adenine Dinucleotide Phosphate) ELISA Kit, 48T

ELK8020-48T, NAADP(Nicotinic Acid Adenine Dinucleotide Phosphate) ELISA Kit, 48T

ELK8021-48T, Rat EML2(Echinoderm Microtubule Associated Protein Like Protein 2) ELISA Kit, 48T

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ELK8020-96T, NAADP(Nicotinic Acid Adenine Dinucleotide Phosphate) ELISA Kit, 96T

2.475,20 RON

NAADP(Nicotinic Acid Adenine Dinucleotide Phosphate) ELISA Kit

SKU
ELK8020-96T

Alternative Names: NAADP

Species: General

Assay Type: Competitive Inhibition

Sensitivity: 48.7 ng/mL

Standard: 10000 ng/mL

Detection range: 156.25-10000 ng/mL

Sample type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length: 2h

Research Area: Signal transduction;Enzyme & Kinase;Metabolic pathway;Tumor immunity;

Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with NAADP protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to NAADP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of NAADP in the samples is then determined by comparing the OD of the samples to the standard curve.

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Description

Alternative Names: NAADP

Species: General

Assay Type: Competitive Inhibition

Sensitivity: 48.7 ng/mL

Standard: 10000 ng/mL

Detection range: 156.25-10000 ng/mL

Sample type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length: 2h

Research Area: Signal transduction;Enzyme & Kinase;Metabolic pathway;Tumor immunity;

Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with NAADP protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to NAADP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of NAADP in the samples is then determined by comparing the OD of the samples to the standard curve.