ELK7999-48T, Cattle PEDF(Pigment Epithelium Derived Factor) ELISA Kit, 48T

Alternative Names: SERPINF1; EPC1; PIG35; SDF3; Serpin F1; Cell proliferation-inducing gene 35; Serpin Peptidase Inhibitor,Clade F Member 1(Alpha-2 Antiplasmin); Stromal Cell Derived Factor 3

Species: Cattle

Assay Type: Sandwich

Sensitivity: 0.135 ng/mL

Standard: 20 ng/mL

Detection range: 0.32-20 ng/mL

Sample type: Serum, plasma and other biological fluids.

Assay length: 3.5h

Research Area: Cytokine;Neuro science;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle PEDF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle PEDF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle PEDF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle PEDF in the samples is then determined by comparing the OD of the samples to the standard curve.