ELK7984-48T, VD(Vitamin D) ELISA Kit, 48T

Alternative Names: VD; VD1; Lamisterol; Vitamin D; Vitamin D1; Lumisterol

Species: General

Assay Type: Competitive Inhibition

Sensitivity: 2.19 ng/mL

Standard: 400 ng/mL

Detection range: 6.25-400 ng/mL

Sample type: Serum, plasma and other biological fluids

Assay length: 2h

Research Area: Metabonomics

Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with VD protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to VD. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of VD in the samples is then determined by comparing the OD of the samples to the standard curve.