ELK7921-48T, HVA(Homovanillic Acid) ELISA Kit, 48T

ELK7921-48T, HVA(Homovanillic Acid) ELISA Kit, 48T

ELK7922-48T, Pig FABP1(Fatty Acid Binding Protein 1, Liver) ELISA Kit, 48T

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ELK7921-96T, HVA(Homovanillic Acid) ELISA Kit, 96T

2.475,20 RON

HVA(Homovanillic Acid) ELISA Kit

SKU
ELK7921-96T

Alternative Names: HOC6H3(OCH3)CH2COOH; 3-Methoxy-4-hydroxyphenyl Acetic Acid; 4-Hydroxy-3-Methoxy-Benzeneacetic Acid; 4-Hydroxy-3-Methoxyphenylacetic Acid

Species: General

Assay Type: Competitive Inhibition

Sensitivity: 0.97 ng/mL

Standard: 200 ng/mL

Detection range: 3.13-200 ng/mL

Sample type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length: 2h

Research Area: Neuroscience

Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with HVA protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to HVA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of HVA in the samples is then determined by comparing the OD of the samples to the standard curve.

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Description

Alternative Names: HOC6H3(OCH3)CH2COOH; 3-Methoxy-4-hydroxyphenyl Acetic Acid; 4-Hydroxy-3-Methoxy-Benzeneacetic Acid; 4-Hydroxy-3-Methoxyphenylacetic Acid

Species: General

Assay Type: Competitive Inhibition

Sensitivity: 0.97 ng/mL

Standard: 200 ng/mL

Detection range: 3.13-200 ng/mL

Sample type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length: 2h

Research Area: Neuroscience

Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with HVA protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to HVA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of HVA in the samples is then determined by comparing the OD of the samples to the standard curve.