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ELK7917-96T, Pig sC5b-9(Soluble Terminal Complement Complex C5b-9) ELISA Kit, 96T
3.736,60 RON
Pig sC5b-9(Soluble Terminal Complement Complex C5b-9) ELISA Kit
SKU
ELK7917-96T
Alternative Names: MAC; Membrane Attack Complex; C5b-9; Terminal Complement Complex C5b-9; C5b9
Species: Pig
Assay Type: Sandwich
Sensitivity: 0.32 ng/mL
Standard: 50 ng/mL
Detection range: 0.79-50 ng/mL
Sample type: Plasma, tissue homogenates and other biological fluids
Assay length: 3.5h
Research Area: Signal transduction;Metabolic pathway;Apoptosis;Infection immunity;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pig sC5b-9. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig sC5b-9. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pig sC5b-9, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig sC5b-9 in the samples is then determined by comparing the OD of the samples to the standard curve.
| Price | 3.140,00 RON (preturile sunt fara TVA) |
|---|---|
| Description |
Alternative Names: MAC; Membrane Attack Complex; C5b-9; Terminal Complement Complex C5b-9; C5b9 Species: Pig Assay Type: Sandwich Sensitivity: 0.32 ng/mL Standard: 50 ng/mL Detection range: 0.79-50 ng/mL Sample type: Plasma, tissue homogenates and other biological fluids Assay length: 3.5h Research Area: Signal transduction;Metabolic pathway;Apoptosis;Infection immunity; Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pig sC5b-9. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig sC5b-9. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pig sC5b-9, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig sC5b-9 in the samples is then determined by comparing the OD of the samples to the standard curve. |
