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ELK7898-96T, rT3(Reverse Triiodothyronine) ELISA Kit, 96T
2.475,20 RON
rT3(Reverse Triiodothyronine) ELISA Kit
SKU
ELK7898-96T
Alternative Names: RT3; r-T3; Reverse T3; (2S)-2-Amino-3-[4-(4-Hydroxy-3,5-Diiodophenoxy)-3-Iodophenyl]propanoic Acid; 3,3',5'-Triiodo-L-thyronine
Species: General
Assay Type: Competitive Inhibition
Sensitivity: 27.55 pg/mL
Standard: 6000 pg/mL
Detection range: 93.75-6000 pg/mL
Sample type: serum, plasma and other biological fluids
Assay length: 2h
Research Area: Infection immunity;Endocrinology;Hormone metabolism;Autoimmunity;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with rT3 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to rT3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of rT3 in the samples is then determined by comparing the OD of the samples to the standard curve.
| Price | 2.080,00 RON (preturile sunt fara TVA) |
|---|---|
| Description |
Alternative Names: RT3; r-T3; Reverse T3; (2S)-2-Amino-3-[4-(4-Hydroxy-3,5-Diiodophenoxy)-3-Iodophenyl]propanoic Acid; 3,3',5'-Triiodo-L-thyronine Species: General Assay Type: Competitive Inhibition Sensitivity: 27.55 pg/mL Standard: 6000 pg/mL Detection range: 93.75-6000 pg/mL Sample type: serum, plasma and other biological fluids Assay length: 2h Research Area: Infection immunity;Endocrinology;Hormone metabolism;Autoimmunity; Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with rT3 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to rT3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of rT3 in the samples is then determined by comparing the OD of the samples to the standard curve. |
