ELK7888-96T, ACH(Acetylcholine) ELISA Kit, 96T

ELK7888-96T, ACH(Acetylcholine) ELISA Kit, 96T

ELK7889-96T, VB6(Vitamin B6) ELISA Kit, 96T

ELK7889-96T, VB6(Vitamin B6) ELISA Kit, 96T

ELK7889-48T, VB6(Vitamin B6) ELISA Kit, 48T

1.814,75 RON

VB6(Vitamin B6) ELISA Kit

SKU
ELK7889-48T

Alternative Names: Pyridoxine

Species: General

Assay Type: Competitive Inhibition

Sensitivity: 1.42 ng/mL

Standard: 300 ng/mL

Detection range: 4.69-300 ng/mL

Sample type: serum, plasma and other biological fluids

Assay length: 2h

Research Area: Nutrition metabolism;Hormone metabolism;

Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with VB6 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to VB6. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of VB6 in the samples is then determined by comparing the OD of the samples to the standard curve.

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Description

Alternative Names: Pyridoxine

Species: General

Assay Type: Competitive Inhibition

Sensitivity: 1.42 ng/mL

Standard: 300 ng/mL

Detection range: 4.69-300 ng/mL

Sample type: serum, plasma and other biological fluids

Assay length: 2h

Research Area: Nutrition metabolism;Hormone metabolism;

Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with VB6 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to VB6. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of VB6 in the samples is then determined by comparing the OD of the samples to the standard curve.