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ELK7866-48T, 3-NT(3-Nitrotyrosine) ELISA Kit, 48T
1.814,75 RON
3-NT(3-Nitrotyrosine) ELISA Kit
SKU
ELK7866-48T
Alternative Names: 3-Nitro-L-Tyrosine; 3-Nitrotyrosine; Nitrotyrosine
Species: General
Assay Type: Competitive Inhibition
Sensitivity: 1.35 ng/mL
Standard: 300 ng/mL
Detection range: 4.69-300 ng/mL
Sample type: serum, plasma and other biological fluids
Assay length: 2h
Research Area: Metabolic pathway;Infection immunity;Rheumatology;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 3-NT protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 3-NT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 3-NT in the samples is then determined by comparing the OD of the samples to the standard curve.
| Price | 1.525,00 RON (preturile sunt fara TVA) |
|---|---|
| Description |
Alternative Names: 3-Nitro-L-Tyrosine; 3-Nitrotyrosine; Nitrotyrosine Species: General Assay Type: Competitive Inhibition Sensitivity: 1.35 ng/mL Standard: 300 ng/mL Detection range: 4.69-300 ng/mL Sample type: serum, plasma and other biological fluids Assay length: 2h Research Area: Metabolic pathway;Infection immunity;Rheumatology; Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 3-NT protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 3-NT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 3-NT in the samples is then determined by comparing the OD of the samples to the standard curve. |
