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ELK7844-48T, Human Anti-IAA(Anti-Insulin Autoantibody Antibody) ELISA Kit, 48T
2.142,00 RON
Human Anti-IAA(Anti-Insulin Autoantibody Antibody) ELISA Kit
SKU
ELK7844-48T
Alternative Names: Anti-Insulin; Antiinsulin; Insulin Antibodies
Species: Human
Assay Type: Competitive Inhibition
Sensitivity: 1.15 ng/mL
Standard: 200 ng/mL
Detection range: 3.13-200 ng/mL
Sample type: Serum, plasma and other biological fluids.
Assay length: 2.5h
Research Area: Signal transduction;Endocrinology;Autoimmunity;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human Anti-IAA protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Anti-IAA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Anti-IAA in the samples is then determined by comparing the OD of the samples to the standard curve.
| Price | 1.800,00 RON (preturile sunt fara TVA) |
|---|---|
| Description |
Alternative Names: Anti-Insulin; Antiinsulin; Insulin Antibodies Species: Human Assay Type: Competitive Inhibition Sensitivity: 1.15 ng/mL Standard: 200 ng/mL Detection range: 3.13-200 ng/mL Sample type: Serum, plasma and other biological fluids. Assay length: 2.5h Research Area: Signal transduction;Endocrinology;Autoimmunity; Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human Anti-IAA protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Anti-IAA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Anti-IAA in the samples is then determined by comparing the OD of the samples to the standard curve. |
