ELK7834-48T, FA(Folic Acid) ELISA Kit, 48T

ELK7834-48T, FA(Folic Acid) ELISA Kit, 48T

ELK7835-48T, Hyp(Hydroxyproline) ELISA Kit, 48T

ELK7835-48T, Hyp(Hydroxyproline) ELISA Kit, 48T

ELK7834-96T, FA(Folic Acid) ELISA Kit, 96T

2.475,20 RON

FA(Folic Acid) ELISA Kit

SKU
ELK7834-96T

Alternative Names: VB9; Vitamin B9; Folacin; Folate; V; Itamin M; Vitamin M; Vitamin Bc; Pteroyl-L-Glutamic Acid; Pteroyl-L-Glutamate

Species: General

Assay Type: Competitive Inhibition

Sensitivity: 14.7 pg/mL

Standard: 10000 pg/mL

Detection range: 156.25-10000 pg/mL

Sample type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length: 2h

Research Area: Hematology;Reproductive science;Nutrition metabolism;

Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with FA protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to FA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of FA in the samples is then determined by comparing the OD of the samples to the standard curve.

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Description

Alternative Names: VB9; Vitamin B9; Folacin; Folate; V; Itamin M; Vitamin M; Vitamin Bc; Pteroyl-L-Glutamic Acid; Pteroyl-L-Glutamate

Species: General

Assay Type: Competitive Inhibition

Sensitivity: 14.7 pg/mL

Standard: 10000 pg/mL

Detection range: 156.25-10000 pg/mL

Sample type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length: 2h

Research Area: Hematology;Reproductive science;Nutrition metabolism;

Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with FA protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to FA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of FA in the samples is then determined by comparing the OD of the samples to the standard curve.