MDA(Malondialdehyde) ELISA Kit
Alternative Names: MDA
Species: General
Assay Type: Competitive Inhibition
Sensitivity: 9.15 ng/mL
Standard: 2000 ng/mL
Detection range: 31.25-2000 ng/mL
Sample type: serum, plasma and other biological fluids
Assay length: 2h
Research Area: Metabolic pathway;Hepatology;Hormone metabolism;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with MDA protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to MDA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of MDA in the samples is then determined by comparing the OD of the samples to the standard curve.
Price | 2.080,00 RON (preturile sunt fara TVA) |
---|---|
Description |
Alternative Names: MDA Species: General Assay Type: Competitive Inhibition Sensitivity: 9.15 ng/mL Standard: 2000 ng/mL Detection range: 31.25-2000 ng/mL Sample type: serum, plasma and other biological fluids Assay length: 2h Research Area: Metabolic pathway;Hepatology;Hormone metabolism; Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with MDA protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to MDA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of MDA in the samples is then determined by comparing the OD of the samples to the standard curve. |