ELK7807-96T, Chicken BAFF/CD257(B-Cell Activating Factor) ELISA Kit, 96T

Alternative Names: CD257; TNFSF13B; BLYS; TALL1; THANK; TNFSF20; ZTNF4; B lymphocyte stimulator; Tumor Necrosis Factor Ligand Superfamily Member 13B; Dendritic cell-derived TNF-like; BAFF

Species: Chicken

Assay Type: Sandwich

Sensitivity: 27 pg/mL

Standard: 5000 pg/mL

Detection range: 78.13-5000 pg/mL

Sample type: serum, plasma, tissue homogenates, cell lysates and other biological fluids

Assay length: 3.5h

Research Area: CD & Adhesion molecule;Tumor immunity;Infection immunity;Immune molecule;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chicken BAFF/CD257. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Chicken BAFF/CD257. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Chicken BAFF/CD257, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Chicken BAFF/CD257 in the samples is then determined by comparing the OD of the samples to the standard curve.