ELK7788-48T, Mouse HMWK(High Molecular Weight Kininogen) ELISA Kit, 48T

ELK7788-48T, Mouse HMWK(High Molecular Weight Kininogen) ELISA Kit, 48T

ELK7789-48T, Mouse SYN2(Synapsin II) ELISA Kit, 48T

ELK7789-48T, Mouse SYN2(Synapsin II) ELISA Kit, 48T

ELK7788-96T, Mouse HMWK(High Molecular Weight Kininogen) ELISA Kit, 96T

2.963,10 RON

Mouse HMWK(High Molecular Weight Kininogen) ELISA Kit

SKU
ELK7788-96T

Alternative Names: Williams-Fitzgerald-Flaujeac Factor; Fitzgerald Factor; HMWK-Kallikrein Factor

Species: Mouse

Assay Type: Sandwich

Sensitivity: 0.288 ng/mL

Standard: 40 ng/mL

Detection range: 0.63-40 ng/mL

Sample type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length: 3.5h

Research Area: Enzyme & Kinase;Hematology;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse HMWK. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse HMWK. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse HMWK, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse HMWK in the samples is then determined by comparing the OD of the samples to the standard curve.

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Description

Alternative Names: Williams-Fitzgerald-Flaujeac Factor; Fitzgerald Factor; HMWK-Kallikrein Factor

Species: Mouse

Assay Type: Sandwich

Sensitivity: 0.288 ng/mL

Standard: 40 ng/mL

Detection range: 0.63-40 ng/mL

Sample type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length: 3.5h

Research Area: Enzyme & Kinase;Hematology;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse HMWK. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse HMWK. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse HMWK, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse HMWK in the samples is then determined by comparing the OD of the samples to the standard curve.