ELK7733-48T, Rat ACR(Acrosin) ELISA Kit, 48T

ELK7733-48T, Rat ACR(Acrosin) ELISA Kit, 48T

ELK7734-48T, Mouse HIST1H4A(Histone Cluster 1, H4a) ELISA Kit, 48T

ELK7734-48T, Mouse HIST1H4A(Histone Cluster 1, H4a) ELISA Kit, 48T

ELK7733-96T, Rat ACR(Acrosin) ELISA Kit, 96T

2.963,10 RON

Rat ACR(Acrosin) ELISA Kit

SKU
ELK7733-96T

Alternative Names: ACRS; Preproacrosin

Species: Rat

Assay Type: Sandwich

Sensitivity: 0.061 ng/mL

Standard: 10 ng/mL

Detection range: 0.16-10 ng/mL

Sample type: tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length: 3.5h

Research Area: Reproductive science;Developmental science;Nutrition metabolism;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat ACR. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat ACR. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat ACR, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat ACR in the samples is then determined by comparing the OD of the samples to the standard curve.

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Description

Alternative Names: ACRS; Preproacrosin

Species: Rat

Assay Type: Sandwich

Sensitivity: 0.061 ng/mL

Standard: 10 ng/mL

Detection range: 0.16-10 ng/mL

Sample type: tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length: 3.5h

Research Area: Reproductive science;Developmental science;Nutrition metabolism;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat ACR. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat ACR. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat ACR, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat ACR in the samples is then determined by comparing the OD of the samples to the standard curve.