ELK7701-96T, Rat aHSP(Alpha-Hemoglobin Stabilizing Protein) ELISA Kit, 96T

Alternative Names: A-HSP; ERAF; EDRF; Erythroid Associated Factor; Erythroid differentiation-related factor

Species: Rat

Assay Type: Sandwich

Sensitivity: 0.57 ng/mL

Standard: 100 ng/mL

Detection range: 1.57-100 ng/mL

Sample type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length: 3.5h

Research Area: Metabolic pathway;Infection immunity;Cardiovascular biology;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat aHSP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat aHSP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat aHSP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat aHSP in the samples is then determined by comparing the OD of the samples to the standard curve.