ELK7677-96T, Rat Ang1-7(Angiotensin 1-7) ELISA Kit, 96T

ELK7677-96T, Rat Ang1-7(Angiotensin 1-7) ELISA Kit, 96T

ELK7678-96T, Human Ang1-7(Angiotensin 1-7) ELISA Kit, 96T

ELK7678-96T, Human Ang1-7(Angiotensin 1-7) ELISA Kit, 96T

ELK7678-48T, Human Ang1-7(Angiotensin 1-7) ELISA Kit, 48T

2.142,00 RON

Human Ang1-7(Angiotensin 1-7) ELISA Kit

SKU
ELK7678-48T

Alternative Names: Ang1-7

Species: Human

Assay Type: Sandwich

Sensitivity: 4.69 pg/mL

Standard: 1000 pg/mL

Detection range: 15.63-1000 pg/mL

Sample type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length: 3.5h

Research Area: Endocrinology;Cardiovascular biology;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Ang1-7. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Ang1-7. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human Ang1-7, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Ang1-7 in the samples is then determined by comparing the OD of the samples to the standard curve.

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Description

Alternative Names: Ang1-7

Species: Human

Assay Type: Sandwich

Sensitivity: 4.69 pg/mL

Standard: 1000 pg/mL

Detection range: 15.63-1000 pg/mL

Sample type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length: 3.5h

Research Area: Endocrinology;Cardiovascular biology;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Ang1-7. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Ang1-7. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human Ang1-7, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Ang1-7 in the samples is then determined by comparing the OD of the samples to the standard curve.