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ELK7659-48T, Mouse IgM(Immunoglobulin M) ELISA Kit, 48T
2.142,00 RON
Mouse IgM(Immunoglobulin M) ELISA Kit
SKU
ELK7659-48T
Alternative Names: IGHM; Immunoglobulin Heavy Constant Mu; Ig mu chain C region
Species: Mouse
Assay Type: Sandwich
Sensitivity: 1.35 µg/mL
Standard: 200 µg/mL
Detection range: 3.13-200 µg/mL
Sample type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length: 3.5h
Research Area: Infection immunity;Immune molecule;Hematology;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse IgM. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse IgM. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse IgM, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse IgM in the samples is then determined by comparing the OD of the samples to the standard curve.
| Price | 1.800,00 RON (preturile sunt fara TVA) |
|---|---|
| Description |
Alternative Names: IGHM; Immunoglobulin Heavy Constant Mu; Ig mu chain C region Species: Mouse Assay Type: Sandwich Sensitivity: 1.35 µg/mL Standard: 200 µg/mL Detection range: 3.13-200 µg/mL Sample type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids Assay length: 3.5h Research Area: Infection immunity;Immune molecule;Hematology; Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse IgM. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse IgM. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse IgM, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse IgM in the samples is then determined by comparing the OD of the samples to the standard curve. |
