ELK7626-48T, Mouse VASH1(Vasohibin 1) ELISA Kit, 48T

ELK7626-48T, Mouse VASH1(Vasohibin 1) ELISA Kit, 48T

ELK7627-48T, Human MOTS-c(Mitochondrial Open Reading Frame Of The 12S rRNA-c) ELISA Kit, 48T

ELK7627-48T, Human MOTS-c(Mitochondrial Open Reading Frame Of The 12S rRNA-c) ELISA Kit, 48T

ELK7626-96T, Mouse VASH1(Vasohibin 1) ELISA Kit, 96T

2.963,10 RON

Mouse VASH1(Vasohibin 1) ELISA Kit

SKU
ELK7626-96T

Alternative Names: Vash1; Vash; Vasohibin-1

Species: Mouse

Assay Type: Sandwich

Sensitivity: 0.29 ng/mL

Standard: 50 ng/mL

Detection range: 0.79-50 ng/mL

Sample type: Tissue homogenates, cell lysates and other biological fluids

Assay length: 3.5h

Research Area: Cardiovascular biology;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse VASH1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse VASH1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse VASH1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse VASH1 in the samples is then determined by comparing the OD of the samples to the standard curve.

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Description

Alternative Names: Vash1; Vash; Vasohibin-1

Species: Mouse

Assay Type: Sandwich

Sensitivity: 0.29 ng/mL

Standard: 50 ng/mL

Detection range: 0.79-50 ng/mL

Sample type: Tissue homogenates, cell lysates and other biological fluids

Assay length: 3.5h

Research Area: Cardiovascular biology;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse VASH1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse VASH1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse VASH1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse VASH1 in the samples is then determined by comparing the OD of the samples to the standard curve.