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ELK7582-96T, Human CYP4F2(Cytochrome P450 4F2) ELISA Kit, 96T
2.963,10 RON
Human CYP4F2(Cytochrome P450 4F2) ELISA Kit
SKU
ELK7582-96T
Alternative Names: CPF2; Leukotriene-B(4)omega-hydroxylase 1; Cytochrome P450 Family 4 Subfamily F Polypeptide 2; 20-hydroxyeicosatetraenoic acid synthase; Arachidonic acid omega-hydroxylase
Species: Human
Assay Type: Sandwich
Sensitivity: 0.55 ng/mL
Standard: 100 ng/mL
Detection range: 1.57-100 ng/mL
Sample type: tissue homogenates, cell lysates and other biological fluids
Assay length: 3.5h
Research Area: Enzyme & Kinase;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CYP4F2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CYP4F2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CYP4F2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CYP4F2 in the samples is then determined by comparing the OD of the samples to the standard curve.
| Price | 2.490,00 RON (preturile sunt fara TVA) |
|---|---|
| Description |
Alternative Names: CPF2; Leukotriene-B(4)omega-hydroxylase 1; Cytochrome P450 Family 4 Subfamily F Polypeptide 2; 20-hydroxyeicosatetraenoic acid synthase; Arachidonic acid omega-hydroxylase Species: Human Assay Type: Sandwich Sensitivity: 0.55 ng/mL Standard: 100 ng/mL Detection range: 1.57-100 ng/mL Sample type: tissue homogenates, cell lysates and other biological fluids Assay length: 3.5h Research Area: Enzyme & Kinase; Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CYP4F2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CYP4F2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CYP4F2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CYP4F2 in the samples is then determined by comparing the OD of the samples to the standard curve. |
