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ELK7540-96T, Rat CYBb(Cytochrome b-245 Beta Polypeptide) ELISA Kit, 96T
2.963,10 RON
Rat CYBb(Cytochrome b-245 Beta Polypeptide) ELISA Kit
SKU
ELK7540-96T
Alternative Names: CGD; GP91PHOX; NOX2; Neutrophil cytochrome b91; Cytochrome b558 beta; Heme-binding membrane glycoprotein gp91phox; Superoxide-generating NADPH oxidase heavy chain
Species: Rat
Assay Type: Sandwich
Sensitivity: 0.057 ng/mL
Standard: 10 ng/mL
Detection range: 0.16-10 ng/mL
Sample type: Tissue homogenates, cell lysates and other biological fluids
Assay length: 3.5h
Research Area: Metabolic pathway;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat CYBb. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat CYBb. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat CYBb, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat CYBb in the samples is then determined by comparing the OD of the samples to the standard curve.
| Price | 2.490,00 RON (preturile sunt fara TVA) |
|---|---|
| Description |
Alternative Names: CGD; GP91PHOX; NOX2; Neutrophil cytochrome b91; Cytochrome b558 beta; Heme-binding membrane glycoprotein gp91phox; Superoxide-generating NADPH oxidase heavy chain Species: Rat Assay Type: Sandwich Sensitivity: 0.057 ng/mL Standard: 10 ng/mL Detection range: 0.16-10 ng/mL Sample type: Tissue homogenates, cell lysates and other biological fluids Assay length: 3.5h Research Area: Metabolic pathway; Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat CYBb. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat CYBb. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat CYBb, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat CYBb in the samples is then determined by comparing the OD of the samples to the standard curve. |
