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ELK7483-96T, Human Smad3(Mothers Against Decapentaplegic Homolog 3) ELISA Kit, 96T
2.475,20 RON
Human Smad3(Mothers Against Decapentaplegic Homolog 3) ELISA Kit
SKU
ELK7483-96T
Alternative Names: JV15-2; MADH3
Species: Human
Assay Type: Sandwich
Sensitivity: 0.239 ng/mL
Standard: 40 ng/mL
Detection range: 0.63-40 ng/mL
Sample type: tissue homogenates, cell lysates and other biological fluids
Assay length: 3.5h
Research Area: Signal transduction;Tumor immunity;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Smad3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Smad3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human Smad3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Smad3 in the samples is then determined by comparing the OD of the samples to the standard curve.
| Price | 2.080,00 RON (preturile sunt fara TVA) |
|---|---|
| Description |
Alternative Names: JV15-2; MADH3 Species: Human Assay Type: Sandwich Sensitivity: 0.239 ng/mL Standard: 40 ng/mL Detection range: 0.63-40 ng/mL Sample type: tissue homogenates, cell lysates and other biological fluids Assay length: 3.5h Research Area: Signal transduction;Tumor immunity; Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Smad3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Smad3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human Smad3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Smad3 in the samples is then determined by comparing the OD of the samples to the standard curve. |
