ELK7450-48T, Rat MSTN(Myostatin) ELISA Kit, 48T

ELK7450-48T, Rat MSTN(Myostatin) ELISA Kit, 48T

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ELK7450-96T, Rat MSTN(Myostatin) ELISA Kit, 96T

2.963,10 RON

Rat MSTN(Myostatin) ELISA Kit

SKU
ELK7450-96T

Alternative Names: GDF8; Growth Differentiation Factor 8

Species: Rat

Assay Type: Competitive Inhibition

Sensitivity: 0.44 ng/mL

Standard: 90 ng/mL

Detection range: 1.41-90 ng/mL

Sample type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length: 2h

Research Area: Cytokine;Metabolic pathway;Bone metabolism;

Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat MSTN protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat MSTN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat MSTN in the samples is then determined by comparing the OD of the samples to the standard curve.

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Description

Alternative Names: GDF8; Growth Differentiation Factor 8

Species: Rat

Assay Type: Competitive Inhibition

Sensitivity: 0.44 ng/mL

Standard: 90 ng/mL

Detection range: 1.41-90 ng/mL

Sample type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length: 2h

Research Area: Cytokine;Metabolic pathway;Bone metabolism;

Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat MSTN protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat MSTN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat MSTN in the samples is then determined by comparing the OD of the samples to the standard curve.