ELK7257-96T, Mouse MYBPC1(Myosin Binding Protein C, Slow Type) ELISA Kit, 96T

Alternative Names: MYBPCC; MYBPCS; C-protein, skeletal muscle slow isoform

Species: Mouse

Assay Type: Sandwich

Sensitivity: 0.06 ng/mL

Standard: 10 ng/mL

Detection range: 0.16-10 ng/mL

Sample type: Serum, plasma, tissue homogenates and other biological fluids.

Assay length: 3.5h

Research Area: Metabolic pathway;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse MYBPC1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse MYBPC1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse MYBPC1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse MYBPC1 in the samples is then determined by comparing the OD of the samples to the standard curve.