ELK7252-96T, Human IFIH1(Interferon Induced Helicase C Domain Containing Protein 1) ELISA Kit, 96T

Alternative Names: Hlcd; IDDM19; MDA5; Helicard; Clinically amyopathic dermatomyositis autoantigen 140 kDa; Melanoma differentiation-associated protein 5; Murabutide down-regulated protein

Species: Human

Assay Type: Sandwich

Sensitivity: 0.115 ng/mL

Standard: 20 ng/mL

Detection range: 0.32-20 ng/mL

Sample type: Tissue homogenates, cell lysates and other biological fluids.

Assay length: 3.5h

Research Area: Signal transduction;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IFIH1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human IFIH1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human IFIH1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IFIH1 in the samples is then determined by comparing the OD of the samples to the standard curve.