ELK7236-48T, Mouse RAPSN(Receptor Associated Protein Of The Synapse) ELISA Kit, 48T

Alternative Names: CMS1D; CMS1E; RNF205; Rapsyn; Acetylcholine receptor-associated 43 kDa protein; 43 kDa postsynaptic protein; RING finger protein 205

Species: Mouse

Assay Type: Sandwich

Sensitivity: 0.55 ng/mL

Standard: 100 ng/mL

Detection range: 1.57-100 ng/mL

Sample type: Tissue homogenates, cell lysates and other biological fluids.

Assay length: 3.5h

Research Area: Neuro science;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse RAPSN. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse RAPSN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse RAPSN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse RAPSN in the samples is then determined by comparing the OD of the samples to the standard curve.