Dog APOE(Apolipoprotein E) ELISA Kit
Alternative Names: Apo-E; AD2; Apoprotein; Alzheimer Disease 2(E4-Associated,Late Onset
Species: Dog
Assay Type: Sandwich
Sensitivity: 0.65 ng/mL
Standard: 100 ng/mL
Detection range: 1.57-100 ng/mL
Sample type: Serum, plasma and other biological fluids.
Assay length: 3.5h
Research Area: Metabolic pathway;Cardiovascular biology;Hepatology;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dog APOE. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog APOE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dog APOE, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dog APOE in the samples is then determined by comparing the OD of the samples to the standard curve.
Price | 3.140,00 RON (preturile sunt fara TVA) |
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Description |
Alternative Names: Apo-E; AD2; Apoprotein; Alzheimer Disease 2(E4-Associated,Late Onset Species: Dog Assay Type: Sandwich Sensitivity: 0.65 ng/mL Standard: 100 ng/mL Detection range: 1.57-100 ng/mL Sample type: Serum, plasma and other biological fluids. Assay length: 3.5h Research Area: Metabolic pathway;Cardiovascular biology;Hepatology; Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dog APOE. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog APOE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dog APOE, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dog APOE in the samples is then determined by comparing the OD of the samples to the standard curve. |