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ELK7230-96T, Rat MFGE8(Milk Fat Globule EGF Factor 8) ELISA Kit, 96T
2.963,10 RON
Rat MFGE8(Milk Fat Globule EGF Factor 8) ELISA Kit
SKU
ELK7230-96T
Alternative Names: HMFG; MFGM; SED1; BA46; EDIL1; OAcGD3S; SPAG10; hP47; Medin; Lactadherin; Sperm Associated Antigen 10; Sperm Surface Protein hP47; Milk fat globule-EGF factor 8
Species: Rat
Assay Type: Sandwich
Sensitivity: 5.9 pg/mL
Standard: 1000 pg/mL
Detection range: 15.63-1000 pg/mL
Sample type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length: 3.5h
Research Area: Tumor immunity;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat MFGE8. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat MFGE8. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat MFGE8, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat MFGE8 in the samples is then determined by comparing the OD of the samples to the standard curve.
| Price | 2.490,00 RON (preturile sunt fara TVA) |
|---|---|
| Description |
Alternative Names: HMFG; MFGM; SED1; BA46; EDIL1; OAcGD3S; SPAG10; hP47; Medin; Lactadherin; Sperm Associated Antigen 10; Sperm Surface Protein hP47; Milk fat globule-EGF factor 8 Species: Rat Assay Type: Sandwich Sensitivity: 5.9 pg/mL Standard: 1000 pg/mL Detection range: 15.63-1000 pg/mL Sample type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids Assay length: 3.5h Research Area: Tumor immunity; Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat MFGE8. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat MFGE8. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat MFGE8, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat MFGE8 in the samples is then determined by comparing the OD of the samples to the standard curve. |
