ELK7204-48T, Rat SNCa(Synuclein Alpha) ELISA Kit, 48T

Alternative Names: SNC-A; αSYN; PD1; PARK1; PARK4; NACP; Non-A Beta Component Of Alzheimer's Disease Amyloid Precursor Protein; Non-A4 component of amyloid precursor

Species: Rat

Assay Type: Sandwich

Sensitivity: 6.5 pg/mL

Standard: 1000 pg/mL

Detection range: 15.63-1000 pg/mL

Sample type: serum, plasma, tissue homogenates and other biological fluids

Assay length: 3.5h

Research Area: Signal transduction;Neuro science;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat SNCa. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat SNCa. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat SNCa, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat SNCa in the samples is then determined by comparing the OD of the samples to the standard curve.