ELK7179-48T, Human CFHR5(Complement Factor H Related Protein 5) ELISA Kit, 48T

ELK7179-48T, Human CFHR5(Complement Factor H Related Protein 5) ELISA Kit, 48T

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ELK7179-96T, Human CFHR5(Complement Factor H Related Protein 5) ELISA Kit, 96T

2.963,10 RON

Human CFHR5(Complement Factor H Related Protein 5) ELISA Kit

SKU
ELK7179-96T

Alternative Names: CFHL5; FHR-5; FHR5

Species: Human

Assay Type: Sandwich

Sensitivity: 14.8 pg/mL

Standard: 2000 pg/mL

Detection range: 31.25-2000 pg/mL

Sample type: Serum, plasma and other biological fluids.

Assay length: 3.5h

Research Area: Hematology;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CFHR5. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CFHR5. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CFHR5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CFHR5 in the samples is then determined by comparing the OD of the samples to the standard curve.

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Description

Alternative Names: CFHL5; FHR-5; FHR5

Species: Human

Assay Type: Sandwich

Sensitivity: 14.8 pg/mL

Standard: 2000 pg/mL

Detection range: 31.25-2000 pg/mL

Sample type: Serum, plasma and other biological fluids.

Assay length: 3.5h

Research Area: Hematology;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CFHR5. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CFHR5. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CFHR5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CFHR5 in the samples is then determined by comparing the OD of the samples to the standard curve.