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ELK7173-96T, Rat HB(Hemoglobin) ELISA Kit, 96T
2.963,10 RON
Rat HB(Hemoglobin) ELISA Kit
SKU
ELK7173-96T
Alternative Names: Hgb; Haemoglobin; Heterotetramer(αβ)2
Species: Rat
Assay Type: Competitive Inhibition
Sensitivity: 2.59 µg/mL
Standard: 500 µg/mL
Detection range: 7.82-500 µg/mL
Sample type: serum, plasma, tissue homogenates, erythrocyte lysates, cell culture supernates and other biological fluids
Assay length: 2h
Research Area: Metabolic pathway;Infection immunity;Hematology;Neuro science;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat HB protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat HB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat HB in the samples is then determined by comparing the OD of the samples to the standard curve.
| Price | 2.490,00 RON (preturile sunt fara TVA) |
|---|---|
| Description |
Alternative Names: Hgb; Haemoglobin; Heterotetramer(αβ)2 Species: Rat Assay Type: Competitive Inhibition Sensitivity: 2.59 µg/mL Standard: 500 µg/mL Detection range: 7.82-500 µg/mL Sample type: serum, plasma, tissue homogenates, erythrocyte lysates, cell culture supernates and other biological fluids Assay length: 2h Research Area: Metabolic pathway;Infection immunity;Hematology;Neuro science; Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat HB protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat HB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat HB in the samples is then determined by comparing the OD of the samples to the standard curve. |
