ELK7146-48T, Mouse CLDN5(Claudin 5) ELISA Kit, 48T

ELK7146-48T, Mouse CLDN5(Claudin 5) ELISA Kit, 48T

ELK7147-48T, Human LRRC4B(Leucine Rich Repeat Containing Protein 4B) ELISA Kit, 48T

ELK7147-48T, Human LRRC4B(Leucine Rich Repeat Containing Protein 4B) ELISA Kit, 48T

ELK7146-96T, Mouse CLDN5(Claudin 5) ELISA Kit, 96T

2.963,10 RON

Mouse CLDN5(Claudin 5) ELISA Kit

SKU
ELK7146-96T

Alternative Names: AWAL; BEC1; CPETRL1; TMVCF; Transmembrane Protein Deleted In Velocardiofacial Syndrome; Transmembrane protein deleted in VCFS

Species: Mouse

Assay Type: Sandwich

Sensitivity: 0.115 ng/mL

Standard: 20 ng/mL

Detection range: 0.32-20 ng/mL

Sample type: Tissue homogenates, cell lysates and other biological fluids

Assay length: 3.5h

Research Area: Signal transduction;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse CLDN5. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse CLDN5. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse CLDN5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse CLDN5 in the samples is then determined by comparing the OD of the samples to the standard curve.

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Description

Alternative Names: AWAL; BEC1; CPETRL1; TMVCF; Transmembrane Protein Deleted In Velocardiofacial Syndrome; Transmembrane protein deleted in VCFS

Species: Mouse

Assay Type: Sandwich

Sensitivity: 0.115 ng/mL

Standard: 20 ng/mL

Detection range: 0.32-20 ng/mL

Sample type: Tissue homogenates, cell lysates and other biological fluids

Assay length: 3.5h

Research Area: Signal transduction;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse CLDN5. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse CLDN5. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse CLDN5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse CLDN5 in the samples is then determined by comparing the OD of the samples to the standard curve.