ELK7012-48T, Mouse APLN(Apelin) ELISA Kit, 48T

Alternative Names: XNPEP2; APEL; APJ endogenous ligand

Species: Mouse

Assay Type: Competitive Inhibition

Sensitivity: 35.3 pg/mL

Standard: 8000 pg/mL

Detection range: 125-8000 pg/mL

Sample type: Serum, plasma and other biological fluids.

Assay length: 2h

Research Area: Endocrinology;

Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse APLN protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse APLN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse APLN in the samples is then determined by comparing the OD of the samples to the standard curve.