ELK7008-96T, Rat APBB3(Amyloid Beta Precursor Protein Binding Protein B3) ELISA Kit, 96T

Alternative Names: APB-B3; SRA; FE65L2; FE65-L2; Fe65L2 isoform 484; Alzheimer's beta-amyloid interaction protein; Amyloid beta (A4) protein-binding, family B, member 3, isoform CRA_g

Species: Rat

Assay Type: Sandwich

Sensitivity: 0.062 ng/mL

Standard: 10 ng/mL

Detection range: 0.16-10 ng/mL

Sample type: Tissue homogenates and other biological fluids.

Assay length: 3.5h

Research Area: Neuro science;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat APBB3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat APBB3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat APBB3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat APBB3 in the samples is then determined by comparing the OD of the samples to the standard curve.