ELK6997-96T, Rat MAPK14(Mitogen Activated Protein Kinase 14) ELISA Kit, 96T

Alternative Names: CSBP1; CSBP2; CSPB1; EXIP; Mxi2; PRKM14; PRKM15; RK; SAPK2A; P38; Mitogen-activated protein kinase p38 alpha; Cytokine suppressive anti-inflammatory drug-binding protein

Species: Rat

Assay Type: Sandwich

Sensitivity: 0.43 ng/mL

Standard: 80 ng/mL

Detection range: 1.25-80 ng/mL

Sample type: Tissue homogenates and other biological fluids.

Assay length: 3.5h

Research Area: Signal transduction;Enzyme & Kinase;Tumor immunity;Infection immunity;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat MAPK14. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat MAPK14. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat MAPK14, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat MAPK14 in the samples is then determined by comparing the OD of the samples to the standard curve.