ELK6973-96T, Mouse HSD17b10(17-Beta-Hydroxysteroid Dehydrogenase Type 10) ELISA Kit, 96T

ELK6973-96T, Mouse HSD17b10(17-Beta-Hydroxysteroid Dehydrogenase Type 10) ELISA Kit, 96T

ELK6974-96T, Rat SGPP2(Sphingosine-1-Phosphate Phosphatase 2) ELISA Kit, 96T

ELK6974-96T, Rat SGPP2(Sphingosine-1-Phosphate Phosphatase 2) ELISA Kit, 96T

ELK6974-48T, Rat SGPP2(Sphingosine-1-Phosphate Phosphatase 2) ELISA Kit, 48T

2.142,00 RON

Rat SGPP2(Sphingosine-1-Phosphate Phosphatase 2) ELISA Kit

SKU
ELK6974-48T

Alternative Names: SPP2

Species: Rat

Assay Type: Sandwich

Sensitivity: 0.063 ng/mL

Standard: 10 ng/mL

Detection range: 0.16-10 ng/mL

Sample type: Tissue homogenates and other biological fluids.

Assay length: 3.5h

Research Area: Signal transduction;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat SGPP2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat SGPP2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat SGPP2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat SGPP2 in the samples is then determined by comparing the OD of the samples to the standard curve.

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Description

Alternative Names: SPP2

Species: Rat

Assay Type: Sandwich

Sensitivity: 0.063 ng/mL

Standard: 10 ng/mL

Detection range: 0.16-10 ng/mL

Sample type: Tissue homogenates and other biological fluids.

Assay length: 3.5h

Research Area: Signal transduction;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat SGPP2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat SGPP2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat SGPP2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat SGPP2 in the samples is then determined by comparing the OD of the samples to the standard curve.