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ELK6935-96T, Cattle INHB(Inhibin B) ELISA Kit, 96T
3.736,60 RON
Cattle INHB(Inhibin B) ELISA Kit
SKU
ELK6935-96T
Alternative Names: INH-B
Species: Cattle
Assay Type: Sandwich
Sensitivity: 11.5 pg/mL
Standard: 2000 pg/mL
Detection range: 31.25-2000 pg/mL
Sample type: Serum, plasma and other biological fluids.
Assay length: 3.5h
Research Area: Reproductive science;Genetic science;Hormone metabolism;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle INHB. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle INHB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle INHB, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle INHB in the samples is then determined by comparing the OD of the samples to the standard curve.
| Price | 3.140,00 RON (preturile sunt fara TVA) |
|---|---|
| Description |
Alternative Names: INH-B Species: Cattle Assay Type: Sandwich Sensitivity: 11.5 pg/mL Standard: 2000 pg/mL Detection range: 31.25-2000 pg/mL Sample type: Serum, plasma and other biological fluids. Assay length: 3.5h Research Area: Reproductive science;Genetic science;Hormone metabolism; Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle INHB. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle INHB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle INHB, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle INHB in the samples is then determined by comparing the OD of the samples to the standard curve. |
