ELK6913-48T, Mouse 20S-PSM(20S-Proteasome) ELISA Kit, 48T

ELK6913-48T, Mouse 20S-PSM(20S-Proteasome) ELISA Kit, 48T

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ELK6913-96T, Mouse 20S-PSM(20S-Proteasome) ELISA Kit, 96T

2.963,10 RON

Mouse 20S-PSM(20S-Proteasome) ELISA Kit

SKU
ELK6913-96T

Alternative Names: CP; 20SPSM; 20S Core Particle

Species: Mouse

Assay Type: Competitive Inhibition

Sensitivity: 152.4 ng/mL

Standard: 30000 ng/mL

Detection range: 468.75-30000 ng/mL

Sample type: Serum, plasma and other biological fluids.

Assay length: 2h

Research Area: Enzyme & Kinase;

Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse 20S-PSM protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse 20S-PSM. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse 20S-PSM in the samples is then determined by comparing the OD of the samples to the standard curve.

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Description

Alternative Names: CP; 20SPSM; 20S Core Particle

Species: Mouse

Assay Type: Competitive Inhibition

Sensitivity: 152.4 ng/mL

Standard: 30000 ng/mL

Detection range: 468.75-30000 ng/mL

Sample type: Serum, plasma and other biological fluids.

Assay length: 2h

Research Area: Enzyme & Kinase;

Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse 20S-PSM protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse 20S-PSM. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse 20S-PSM in the samples is then determined by comparing the OD of the samples to the standard curve.