ELK6912-96T, Rat GHRL(Ghrelin) ELISA Kit, 96T

Alternative Names: MTLRP; Preproghrelin; Growth Hormone Secretagogue Receptor Ligand; Appetite-regulating hormone; Growth hormone-releasing peptide; Motilin-related peptide

Species: Rat

Assay Type: Competitive Inhibition

Sensitivity: 10.18 pg/mL

Standard: 2000 pg/mL

Detection range: 31.25-2000 pg/mL

Sample type: Serum, plasma and other biological fluids.

Assay length: 2h

Research Area: Endocrinology;

Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat GHRL protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat GHRL. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat GHRL in the samples is then determined by comparing the OD of the samples to the standard curve.