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ELK6862-96T, Dog HP(Haptoglobin) ELISA Kit, 96T
3.736,60 RON
Dog HP(Haptoglobin) ELISA Kit
SKU
ELK6862-96T
Alternative Names: HP; Hp2-Alpha; Alpha-2-Macroglobulin; Zonulin; Hpt; Haptoglobin
Species: Dog
Assay Type: Sandwich
Sensitivity: 24.5 ng/mL
Standard: 4000 ng/mL
Detection range: 62.5-4000 ng/mL
Sample type: Serum, plasma and other biological fluids.
Assay length: 3.5h
Research Area: Infection immunity;Hematology;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dog HP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog HP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dog HP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dog HP in the samples is then determined by comparing the OD of the samples to the standard curve.
| Price | 3.140,00 RON (preturile sunt fara TVA) |
|---|---|
| Description |
Alternative Names: HP; Hp2-Alpha; Alpha-2-Macroglobulin; Zonulin; Hpt; Haptoglobin Species: Dog Assay Type: Sandwich Sensitivity: 24.5 ng/mL Standard: 4000 ng/mL Detection range: 62.5-4000 ng/mL Sample type: Serum, plasma and other biological fluids. Assay length: 3.5h Research Area: Infection immunity;Hematology; Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dog HP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog HP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dog HP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dog HP in the samples is then determined by comparing the OD of the samples to the standard curve. |
