ELK6831-96T, Rat FPA(Fibrinopeptide A) ELISA Kit, 96T

Alternative Names: FP-A

Species: Rat

Assay Type: Competitive Inhibition

Sensitivity: 136.7 pg/mL

Standard: 30000 pg/mL

Detection range: 468.75-30000 pg/mL

Sample type: Plasma.

Assay length: 2h

Research Area: Signal transduction;Hematology;

Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat FPA protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat FPA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat FPA in the samples is then determined by comparing the OD of the samples to the standard curve.