ELK6826-96T, Rat VCP(Valosin Containing Protein) ELISA Kit, 96T

ELK6826-96T, Rat VCP(Valosin Containing Protein) ELISA Kit, 96T

ELK6827-96T, Mouse CDK8(Cyclin Dependent Kinase 8) ELISA Kit, 96T

ELK6827-96T, Mouse CDK8(Cyclin Dependent Kinase 8) ELISA Kit, 96T

ELK6827-48T, Mouse CDK8(Cyclin Dependent Kinase 8) ELISA Kit, 48T

2.142,00 RON

Mouse CDK8(Cyclin Dependent Kinase 8) ELISA Kit

SKU
ELK6827-48T

Alternative Names: K35; Cell division protein kinase 8; Mediator complex subunit CDK8; Mediator of RNA polymerase II transcription subunit CDK8

Species: Mouse

Assay Type: Sandwich

Sensitivity: 0.053 ng/mL

Standard: 10 ng/mL

Detection range: 0.16-10 ng/mL

Sample type: Tissue homogenates and other biological fluids.

Assay length: 3.5h

Research Area: Tumor immunity;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse CDK8. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse CDK8. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse CDK8, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse CDK8 in the samples is then determined by comparing the OD of the samples to the standard curve.

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Description

Alternative Names: K35; Cell division protein kinase 8; Mediator complex subunit CDK8; Mediator of RNA polymerase II transcription subunit CDK8

Species: Mouse

Assay Type: Sandwich

Sensitivity: 0.053 ng/mL

Standard: 10 ng/mL

Detection range: 0.16-10 ng/mL

Sample type: Tissue homogenates and other biological fluids.

Assay length: 3.5h

Research Area: Tumor immunity;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse CDK8. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse CDK8. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse CDK8, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse CDK8 in the samples is then determined by comparing the OD of the samples to the standard curve.