ELK6813-96T, Rat NME1(Non Metastatic Cells 1, Protein NM23A Expressed In) ELISA Kit, 96T

ELK6813-96T, Rat NME1(Non Metastatic Cells 1, Protein NM23A Expressed In) ELISA Kit, 96T

ELK6814-96T, Rat FAPa(Fibroblast Activation Protein Alpha) ELISA Kit, 96T

ELK6814-96T, Rat FAPa(Fibroblast Activation Protein Alpha) ELISA Kit, 96T

ELK6814-48T, Rat FAPa(Fibroblast Activation Protein Alpha) ELISA Kit, 48T

2.142,00 RON

Rat FAPa(Fibroblast Activation Protein Alpha) ELISA Kit

SKU
ELK6814-48T

Alternative Names: APCE; SIMP; DPPIV; Seprase; 170 kDa melanoma membrane-bound gelatinase; Post-proline cleaving enzyme; Serine integral membrane protease; Surface-expressed protease

Species: Rat

Assay Type: Sandwich

Sensitivity: 0.119 ng/mL

Standard: 20 ng/mL

Detection range: 0.32-20 ng/mL

Sample type: serum, plasma, tissue homogenates and other biological fluids

Assay length: 3.5h

Research Area: Tumor immunity;Dermatology;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat FAPa. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat FAPa. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat FAPa, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat FAPa in the samples is then determined by comparing the OD of the samples to the standard curve.

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Description

Alternative Names: APCE; SIMP; DPPIV; Seprase; 170 kDa melanoma membrane-bound gelatinase; Post-proline cleaving enzyme; Serine integral membrane protease; Surface-expressed protease

Species: Rat

Assay Type: Sandwich

Sensitivity: 0.119 ng/mL

Standard: 20 ng/mL

Detection range: 0.32-20 ng/mL

Sample type: serum, plasma, tissue homogenates and other biological fluids

Assay length: 3.5h

Research Area: Tumor immunity;Dermatology;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat FAPa. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat FAPa. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat FAPa, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat FAPa in the samples is then determined by comparing the OD of the samples to the standard curve.