ELK6776-48T, Mouse VNN3(Vanin 3) ELISA Kit, 48T

ELK6776-48T, Mouse VNN3(Vanin 3) ELISA Kit, 48T

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ELK6776-96T, Mouse VNN3(Vanin 3) ELISA Kit, 96T

2.963,10 RON

Mouse VNN3(Vanin 3) ELISA Kit

SKU
ELK6776-96T

Alternative Names: Vascular non-inflammatory molecule 3

Species: Mouse

Assay Type: Sandwich

Sensitivity: 0.118 ng/mL

Standard: 20 ng/mL

Detection range: 0.32-20 ng/mL

Sample type: Tissue homogenates and other biological fluids.

Assay length: 3.5h

Research Area: Enzyme & Kinase;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse VNN3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse VNN3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse VNN3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse VNN3 in the samples is then determined by comparing the OD of the samples to the standard curve.

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Description

Alternative Names: Vascular non-inflammatory molecule 3

Species: Mouse

Assay Type: Sandwich

Sensitivity: 0.118 ng/mL

Standard: 20 ng/mL

Detection range: 0.32-20 ng/mL

Sample type: Tissue homogenates and other biological fluids.

Assay length: 3.5h

Research Area: Enzyme & Kinase;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse VNN3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse VNN3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse VNN3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse VNN3 in the samples is then determined by comparing the OD of the samples to the standard curve.