ELK6720-48T, Cattle OPN(Osteopontin) ELISA Kit, 48T

Alternative Names: SPP1; BNSP; BSPI; ETA1; Secreted Phosphoprotein 1; Bone Sialoprotein I; Early T-Lymphocyte Activation 1; Nephropontin; Urinary stone protein; Uropontin

Species: Cattle

Assay Type: Sandwich

Sensitivity: 0.55 ng/mL

Standard: 100 ng/mL

Detection range: 1.57-100 ng/mL

Sample type: serum, plasma, tissue homogenates and other biological fluids

Assay length: 3.5h

Research Area: Tumor immunity;Bone metabolism;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle OPN. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle OPN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle OPN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle OPN in the samples is then determined by comparing the OD of the samples to the standard curve.